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polyclonal antihuman il 1ra goat antibody  (R&D Systems)


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    R&D Systems polyclonal antihuman il 1ra goat antibody
    Western-blot analysis of <t>IL-1ra</t> protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.
    Polyclonal Antihuman Il 1ra Goat Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antihuman il 1ra goat antibody/product/R&D Systems
    Average 92 stars, based on 16 article reviews
    polyclonal antihuman il 1ra goat antibody - by Bioz Stars, 2026-05
    92/100 stars

    Images

    1) Product Images from "IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4"

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    Journal:

    doi: 10.1046/j.1365-2249.2002.01685.x

    Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.
    Figure Legend Snippet: Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.

    Techniques Used: Western Blot, Comparison, Control

    IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures
    Figure Legend Snippet: IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures

    Techniques Used: Incubation, Control, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Neutralization

    Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.
    Figure Legend Snippet: Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

    Techniques Used: Staining, Control, Recombinant

    Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.
    Figure Legend Snippet: Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Techniques Used: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.
    Figure Legend Snippet: Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Techniques Used: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes
    Figure Legend Snippet: Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes

    Techniques Used: Derivative Assay

    Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.
    Figure Legend Snippet: Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.

    Techniques Used: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay



    Similar Products

    polyclonal antihuman il 1ra goat antibody  (R&D Systems)
    92
    R&D Systems polyclonal antihuman il 1ra goat antibody
    Western-blot analysis of <t>IL-1ra</t> protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.
    Polyclonal Antihuman Il 1ra Goat Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antihuman il 1ra goat antibody/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    polyclonal antihuman il 1ra goat antibody - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Western Blot, Comparison, Control

    IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Incubation, Control, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Neutralization

    Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Staining, Control, Recombinant

    Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Derivative Assay

    Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.

    Journal:

    Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

    doi: 10.1046/j.1365-2249.2002.01685.x

    Figure Lengend Snippet: Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.

    Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

    Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay